Human papillomavirus detection and typing using a nested-PCR-RFLP assay

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Human papillomavirus detection and typing using a nested-PCR-RFLP assay.

BACKGROUND It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. OBJECTIVES Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to detect and type HPV based on the analysis of L1 gene. METHODS Analysis of published DNA sequence of mucosal HPV types to select sequences of new...

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ژورنال

عنوان ژورنال: The Brazilian Journal of Infectious Diseases

سال: 2011

ISSN: 1413-8670

DOI: 10.1016/s1413-8670(11)70229-x